ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using AKT1 antibody (STJ27474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1 antibody (100-200) (STJ27474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Akt1 antibody (251-351 aa) [SRM5087] (STJ11106868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (STJ112889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AKT1 antibody (350-480) (STJ112889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of PC-12 cells using [KO Validated] AKT1 Rabbit polyclonal antibody (STJ11104319) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1 antibody (380-480) (STJ11104319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Confocal imaging of MCF7 cells using [KO Validated] AKT1 Rabbit monoclonal antibody (STJ11104954, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AKT1 antibody (380-480) [S4954RM] (STJ11104954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunoprecipitation analysis of 300 Mu g extracts from MCF7 cells using 3 Mu g AKT1 Rabbit monoclonal antibody (STJ11101453). Western blot was performed from the immunoprecipitate using AKT1 Rabbit monoclonal antibody (STJ11101453) at a dilution of 1:1000.

Anti-AKT1 antibody (381-480) [S3MR] (STJ11101453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of extracts from wild type (WT) and AKT1 knockout (KO) HeLa cells, using AKT1 antibody (STJ11103457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AKT1 antibody (381-480) [S7MR] (STJ11103457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from 1) 3T3, 2) 293T, 3) Hela cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-AKT1 antibody (400-480 aa) (STJ91540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-AKT1 antibody (95-144 aa) (STJ91542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry with Anti-AKT1 Antibody (STJ500060) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-AKT1 antibody (C-Term) (STJ500060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-AKT1 antibody (C-Term) {Biotin} (STJ500061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-AKT1 antibody (C-Term) {FITC} (STJ500062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$713.48

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STJ73589 (2µg/ml) staining of MCF7 (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence

Anti-AKT1 antibody (Internal) (STJ73589)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Cow

$421.88

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STJ73595 (0. 3µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AKT1 antibody (Internal) (STJ73595)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse

$421.88

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Western blot analysis using Akt1 monoclonal antibody against NIH/3T3 (1) , HeLa (2) , COS7 (3) and Jurkat (4) cell lysate.

Anti-AKT1 antibody [3A3] (STJ97820)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Monkey

$194.40 - $469.80

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Anti-AKT1 nanobody [SAA1080] (STJN000196)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

$510.98 - $2,203.88

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using AKT1/2 Rabbit monoclonal antibody (STJ11103503) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AKT1/2 antibody (381-480) [S3MR] (STJ11103503)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of 3T3 cells using Akt1/3 Polyclonal Antibody diluted at 1：1000

Anti-Akt1/3 antibody (406-455 aa) (STJ91551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates, using AKT1/AKT2/AKT3 Rabbit polyclonal antibody (STJ11105345) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AKT1/AKT2/AKT3 antibody (1-123) (STJ11105345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 25 Mu g extracts of Rat brain cells using 3 Mu g Pan-Akt antibody (STJ11100091). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (STJ11100091) at a dilution of 1:1000.

Anti-AKT1/AKT2/AKT3 antibody (311-480) (STJ11100091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from PC-3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ11104478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-AKT1S1 antibody (150-256) (STJ11104478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of NIH-3T3 cells using AKT1S1/PRAS40 Rabbit polyclonal antibody (STJ27994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT1S1 antibody (150-256) (STJ27994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using AKT1S1/AKT1S1/PRAS40 Rabbit monoclonal antibody (STJ11101861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-AKT1S1 antibody (150-256) [S1MR] (STJ11101861)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Western blot with Anti-AKT1S1 Antibody (STJ502639) of mouse liver. Antibody at 1:500 dilution in buffer.

Anti-AKT1S1 antibody (160-210) (STJ502639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-AKT1S1 antibody (160-210) {Biotin} (STJ502640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-AKT1S1 antibody (160-210) {FITC} (STJ502641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Western blot analysis of various cells using PRAS40 Polyclonal Antibody

Anti-AKT1S1 antibody (207-256 aa) (STJ95215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of U2OS cells using Akt2 Rabbit polyclonal antibody (STJ118023) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-AKT2 antibody (100-200) (STJ118023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293T cells, using AKT2 Rabbit polyclonal antibody (STJ119992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AKT2 antibody (381-481) (STJ119992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using AKT2 antibody (STJ22573) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-AKT2 antibody (400-500) (STJ22573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various cells using Akt2 Polyclonal Antibody diluted at 1：500

Anti-AKT2 antibody (432-481 aa) (STJ91552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ70714 (0. 3µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT2 antibody (C-Term) (STJ70714)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Western blot analysis using Akt2 monoclonal antibody against A431 (1) , Jurkat (2) , HEK293 (3) , A549 (4) , MCF-7 (5) and PC-12 (6) cell lysate.

Anti-AKT2 antibody [1B6] (STJ97821)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Monkey

$194.40 - $469.80

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Anti-AKT2 antibody [SRM14707] (STJ11109437)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using AKT3 Rabbit polyclonal antibody (STJ114775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-AKT3 antibody (90-465) (STJ114775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using AKT3 antibody (STJ11104934) at 1:60000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AKT3 antibody (93-154 aa) [S4934RM] (STJ11104934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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STJ70624 (1µg/ml) staining of HepG2 (A) , Jurkat (B) , Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-AKT3 antibody (Internal) (STJ70624)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

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Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein (1) and human ovary carcinoma tissue lysate (2).

Anti-AKT3 antibody [3B12F3F10/2D2B11B11/2D2B11H5] (STJ97822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates using ALAD Rabbit pAb (STJ110696) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ALAD antibody (1-330) (STJ110696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using ALAD antibody (STJ114271) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALAD antibody (1-330) (STJ114271)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using ALAS1 antibody (STJ115423). Blue: DAPI for nuclear staining.

Anti-ALAS1 antibody (1-250) (STJ115423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ALAS1 antibody (STJ28604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ALAS1 antibody (1-250) (STJ28604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ALAS1 Rabbit monoclonal antibody (STJ11102160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ALAS1 antibody (541-640) [S0MR] (STJ11102160)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of various lysates using ALAS2 Rabbit polyclonal antibody (STJ28605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALAS2 antibody (50-320) (STJ28605)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates, using Albumin Rabbit pAb (STJ22574) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALB antibody (25-609) (STJ22574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HepG2 cells using Albumin Rabbit polyclonal antibody (STJ22575) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALB antibody (25-609) (STJ22575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot detection of Human Serum Albumin in 0.5nl human serum and 5ng ALB Recombinant antigens cell lysates using Human Serum Albumin mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Anti-ALB antibody [1A8-C5-B4] (STJ99077)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis using ALB monoclonal antibody (lane 3, 4) and Human IgG monoclonal antibody (lane 1, 2) against human serum (lane 1, 3) and plasma (lane 2, 4).

Anti-ALB antibody [2H3D1/8F6F9] (STJ97823)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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