| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This mouse vWF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Vwf |
| Gene ID: |
22371 |
| Uniprot ID: |
VWF_MOUSE |
| Specificity: |
This kit recognizes Mouse vWF in samples. No significant cross-reactivity or interference between Mouse vWF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Plasma. Expressed in liver. |
| Post Translational Modifications | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
| Protein Name | Von Willebrand FactorVwf Cleaved Into - Von Willebrand Antigen 2Von Willebrand Antigen Ii |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-140837Reactome: -MMU-216083Reactome: -MMU-354192Reactome: -MMU-354194Reactome: -MMU-372708Reactome: -MMU-430116Reactome: -MMU-5674135Reactome: -MMU-75892Reactome: -MMU-76009 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixLocalized To Storage Granules |
| Alternative CLIA Names | Von Willebrand Factor CLIA kitVwf Cleaved Into - Von Willebrand Antigen 2 CLIA kitVon Willebrand Antigen Ii CLIA kitVwf CLIA kit |
| Specificity | This kit recognizes Mouse vWF in samples. No significant cross-reactivity or interference between Mouse vWF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 97 |
| EDTA plasma(n=8) | 88-100 | 94 |
| Cell culture media(n=8) | 86-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.96 | 2.29 | 9.10 | 0.94 | 2.27 | 9.14 |
| Standard deviation | 0.10 | 0.21 | 1.00 | 0.12 | 0.23 | 0.64 |
| CV (%) | 10.42 | 9.17 | 10.99 | 12.77 | 10.13 | 7.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
