| Host: | HEK293 Cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <1 EU/Mu g |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5×104 cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well) , approximately >80% will adhere after 30 mi |
| Gene Symbol: | Vtn |
| Gene ID: | 22370 |
| Uniprot ID: | VTNC_MOUSE |
| Immunogen Region: | Asp20-Lys478 |
| Immunogen: | Recombinant Mouse Vitronectin/V75/VTN Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Asp20-Lys478) of mouse Vitronectin/V75/VTN (Accession #NP_035837.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | DQESCKGRCTQGFMASKKCQ CDELCTYYQSCCADYMEQCK PQVTRGDVFTMPEDDYWSYD YVEEPKNNTNTGVQPENTSP PGDLNPRTDGTLKPTAFLDP EEQPSTPAPKVEQQEEILRP DTTDQGTPEFPEEELCSGKP FDAFTDLKNGSLFAFRGQYC YELDETAVRPGYPKLIQDVW GIEGPIDAAFTRINCQGKTY LFKGSQYWRFEDGVLDPGYP RNISEGFSGIPDNVDAAFA |
| Post Translational Modifications | Sulfated on tyrosine residues. N- and O-glycosylated. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. |
| Function | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. |
| Protein Name | VitronectinVnS-ProteinSerum-Spreading Factor |
| Database Links | Reactome: R-MMU-2129379Reactome: -MMU-216083Reactome: -MMU-3000170Reactome: -MMU-3000178Reactome: -MMU-977606 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Protein Names | Vitronectin proteinVn proteinS-Protein proteinSerum-Spreading Factor proteinVtn protein |
Information sourced from Uniprot.org
