| Short Description : | Recombinant-Active-Mouse SPARC/Osteonectin-C-10*His protein was developed from mammalian cells and has a target region of C-10*His. For use in research applications. |
| Applications: | ELISA/WB |
| Host: | Mammalian Cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit the cell growth of mink lung epithelial cells (Mv1Lu). The ED50 for this effect is 0.6‑2.4 µg/mL. |
| Gene Symbol: | Sparc |
| Gene ID: | 20692 |
| Uniprot ID: | SPRC_MOUSE |
| Immunogen: | Mus musculus (Mouse) |
| Immunogen Region: | Ala18-Ile302 |
| Function | Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. |
| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-3000178Reactome: -MMU-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative Protein Names | Sparc proteinBasement-Membrane Protein 40 proteinBm-40 proteinOsteonectin proteinOn proteinSecreted Protein Acidic And Rich In Cysteine proteinSparc protein |
Information sourced from Uniprot.org
