| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse Lipocalin-2/NGAL/LCN2-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its ability to bind Iron (III) dihydroxybenzoic acid [Fe (DHBA) 3]. The binding of Fe (DHBA) 3 results in the quenching of Trp fluorescence in recombinant mouse Lipocalin-2. Recombinant mouse Lipocalin-2 can bind >47.7 Mu M of Fe (DHBA) 3 |
| Gene Symbol: |
Lcn2 |
| Gene ID: |
16819 |
| Uniprot ID: |
NGAL_MOUSE |
| Immunogen: |
Recombinant Mouse Lipocalin-2/NGAL Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln21-Asn200) of mouse Lipocalin-2/NGAL (Accession #NP_032517.1) fused with an 8×His tag at the C-terminus. |
| Post Translational Modifications | N-glycosylated. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgalLipocalin-2Oncogene 24p324p3Sv-40-Induced 24p3 ProteinSiderocalin Lcn2P25 |
| Database Links | Reactome: R-MMU-6798695Reactome: -MMU-6799990Reactome: -MMU-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Protein Names | Neutrophil Gelatinase-Associated Lipocalin proteinNgal proteinLipocalin-2 proteinOncogene 24p3 protein24p3 proteinSv-40-Induced 24p3 Protein proteinSiderocalin Lcn2 proteinP25 proteinLcn2 protein |
Information sourced from Uniprot.org
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