• Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.
  • Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.

Mouse Interleukin-5 protein (Recombinant) (STJP000354)

SKU:
STJP000354

£133.50 - £4,686.50
Current Stock:
Host: Insect cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse Interleukin-5-protein was developed from insect cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: The ED (50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg. NA
Gene Symbol: Il5
Gene ID: 16191
Uniprot ID: IL5_MOUSE
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells NA
Immunogen Sequence: MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG NA
Tissue Specificity Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells.
Function Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.
Protein Name Interleukin-5
Il-5
B-Cell Growth Factor Ii
Bcgf-Ii
Cytotoxic T-Lymphocyte Inducer
Eosinophil Differentiation Factor
T-Cell Replacing Factor
Trf
Database Links Reactome: R-MMU-512988
Reactome: -MMU-5673001
Reactome: -MMU-912526
Cellular Localisation Secreted
Alternative Protein Names Interleukin-5 protein
Il-5 protein
B-Cell Growth Factor Ii protein
Bcgf-Ii protein
Cytotoxic T-Lymphocyte Inducer protein
Eosinophil Differentiation Factor protein
T-Cell Replacing Factor protein
Trf protein
Il5 protein
Il-5 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance