Mouse Interleukin-10 protein (Recombinant) (STJP000235)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encodingMouse Interleukin-10 mature chain was expressed in CHO Cells. NA
General Information
| Short Description | Recombinant-Mouse Interleukin-10-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.0. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | ED50 was determined by thedose-dependent (with IL-4) proliferation of murine MC/9 cells, andwas found to be < ng/ml, corresponding to a specific activity of > x units/mg. NA |
Target Information
| Gene Symbol | Il10 |
| Gene ID | 16153 |
| Uniprot ID | IL10_MOUSE |
| Immunogen | Optimized DNA sequence encodingMouse Interleukin-10 mature chain was expressed in CHO Cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MPGSALLCCL LLLTGMRISR GQYSREDNNC THFPVGQSHM LLELRTAFSQ VKTFFQTKDQ LDNILLTDSL MQDFKGYLGC QALSEMIQFY LVEVMPQAEK HGPEIKEHLN SLGEKLKTLR MRLRRCHRFL KCENKSKAVE QVKSDFNKLE DQGVYKAMNE FDIFINCIEA YMMIKMKS NA |
Additional Info
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10Il-10Cytokine Synthesis Inhibitory FactorCsif |
| Database Links | Reactome: R-MMU-6783783 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-10 proteinIl-10 proteinCytokine Synthesis Inhibitory Factor proteinCsif proteinIl10 proteinIl-10 protein |
Information sourced from Uniprot.org