| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Active Recombinant Mouse FGF2 was added during DP cell culture. The immunofluorescence identification showed that 1 ng/mL FGF2 could maintain the cellular characteristics of DP cells. The red part is the fluorescently labeled PDFGRA antibody, and the |
| Gene Symbol: | Fgf2 |
| Gene ID: | 14173 |
| Uniprot ID: | FGF2_MOUSE |
| Immunogen Region: | Ala11-Ser154 |
| Immunogen: | Recombinant Mouse FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala11-Ser154) of mouse FGF2/FGFB (Accession #NP_032032.1) fused with 6×His tag at the C-terminus. |
| Immunogen Sequence: | MVCARHQPGGLCLLLLLLCQ FMEDRSAQAGNCWLRQAKNG RCQVLYKTELSKEECCSTGR LSTSWTEEDVNDNTLFKWMI FNGGAPNCIPCKETCENVDC GPGKKCRMNKKNKPRCVCAP DCSNITWKGPVCGLDGKTYR NECALLKARCKEQPELEVQY QGKCKKTCRDVFCPGSSTCV VDQTNNAYCVTCNRICPEPS SSEQSLCGNDGVTYSSACHL RKATCLLGRSIGLAYEGKC |
| Post Translational Modifications | Phosphorylation at Tyr-81 regulates FGF2 unconventional secretion. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2Fgf-2Basic Fibroblast Growth FactorBfgfHeparin-Binding Growth Factor 2Hbgf-2 |
| Database Links | Reactome: R-MMU-109704Reactome: -MMU-1257604Reactome: -MMU-190322Reactome: -MMU-190370Reactome: -MMU-190372Reactome: -MMU-190373Reactome: -MMU-190375Reactome: -MMU-190377Reactome: -MMU-3000170Reactome: -MMU-5654219Reactome: -MMU-5654221Reactome: -MMU-5654227Reactome: -MMU-5654228Reactome: -MMU-5654687Reactome: -MMU-5654688Reactome: -MMU-5654689Reactome: -MMU-5654693Reactome: -MMU-5654695Reactome: -MMU-5654699Reactome: -MMU-5654700Reactome: -MMU-5654704Reactome: -MMU-5654706Reactome: -MMU-5654710Reactome: -MMU-5654712Reactome: -MMU-5654719Reactome: -MMU-5654720Reactome: -MMU-5654726Reactome: -MMU-5654727Reactome: -MMU-5654732Reactome: -MMU-5654733Reactome: -MMU-5658623Reactome: -MMU-5673001Reactome: -MMU-6811558Reactome: -MMU-9839397 |
| Cellular Localisation | SecretedNucleusExported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent MechanismUnconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma MembraneBinding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Cep57 |
| Alternative Protein Names | Fibroblast Growth Factor 2 proteinFgf-2 proteinBasic Fibroblast Growth Factor proteinBfgf proteinHeparin-Binding Growth Factor 2 proteinHbgf-2 proteinFgf2 proteinFgf-2 protein |
Information sourced from Uniprot.org