| Post Translational Modifications | The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. Can be either O-glucosylated or O-xylosylated at Ser-93 by POGLUT1. |
| Function | Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. |
| Protein Name | Coagulation Factor ViiSerum Prothrombin Conversion Accelerator Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain |
| Database Links | Reactome: R-MMU-140834Reactome: -MMU-159740Reactome: -MMU-159763Reactome: -MMU-159782 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Coagulation Factor Vii ELISA kitSerum Prothrombin Conversion Accelerator Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain ELISA kitF7 ELISA kitCf7 ELISA kit |
| Specificity | This kit recognizes Mouse F7 in samples. No significant cross-reactivity or interference between Mouse F7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-99 | 92 |
| EDTA plasma (n=8) | 95-110 | 100 |
| Cell culture media (n=8) | 86-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.07 | 4.70 | 0.48 | 1.06 | 4.76 |
| Standard deviation | 0.03 | 0.05 | 0.24 | 0.03 | 0.06 | 0.16 |
| CV (%) | 6.00 | 4.67 | 5.11 | 6.25 | 5.66 | 3.36 |
