Mouse Dkk-3 protein (Recombinant) (No-Tag) (STJP003049)

SKU:
STJP003049
£97.50 - £291.50
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Host: E.coli
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: <0.01 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 9.66-38.64 Mu g/mL, corresponding to a specific activity of 2.59×101~1.04×102 units/mg.
Gene Symbol: Dkk3
Gene ID: 50781
Uniprot ID: DKK3_MOUSE
Immunogen Region: Ala 22-Ile 349
Immunogen: Recombinant Mouse Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 22-Ile 349) of Mouse Dkk-3 (Accession #NP_056629.1) fused with a His tag at the C-terminus.
Immunogen Sequence: CNDMSPEQTATSVNCSSPER HTRSYDYMEGGDIRVRRLFC RTQWYLRIDKRGKVKGTQEM KNSYNIMEIRTVAVGIVAIK GVESEYYLAMNKEGKLYAKK ECNEDCNFKELILENHYNTY ASAKWTHSGGEMFVALNQKG IPVKGKKTKKEQKTAHFLPM AIT
Tissue Specificity Highest expression in brain, eye and heart.
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Protein Name Dickkopf-Related Protein 3
Dickkopf-3
Dkk-3
Mdkk-3
Cellular Localisation Secreted
Alternative Protein Names Dickkopf-Related Protein 3 protein
Dickkopf-3 protein
Dkk-3 protein
Mdkk-3 protein
Dkk3 protein

Information sourced from Uniprot.org