| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.01 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 9.66-38.64 Mu g/mL, corresponding to a specific activity of 2.59×101~1.04×102 units/mg. |
| Gene Symbol: | Dkk3 |
| Gene ID: | 50781 |
| Uniprot ID: | DKK3_MOUSE |
| Immunogen Region: | Ala 22-Ile 349 |
| Immunogen: | Recombinant Mouse Dkk-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala 22-Ile 349) of Mouse Dkk-3 (Accession #NP_056629.1) fused with a His tag at the C-terminus. |
| Immunogen Sequence: | CNDMSPEQTATSVNCSSPER HTRSYDYMEGGDIRVRRLFC RTQWYLRIDKRGKVKGTQEM KNSYNIMEIRTVAVGIVAIK GVESEYYLAMNKEGKLYAKK ECNEDCNFKELILENHYNTY ASAKWTHSGGEMFVALNQKG IPVKGKKTKKEQKTAHFLPM AIT |
| Tissue Specificity | Highest expression in brain, eye and heart. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Protein Name | Dickkopf-Related Protein 3Dickkopf-3Dkk-3Mdkk-3 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 3 proteinDickkopf-3 proteinDkk-3 proteinMdkk-3 proteinDkk3 protein |
Information sourced from Uniprot.org