| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse CD27 (TNFRSF7)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability of immobilized recombinant Mouse CD27 to bind Human CD70 with a linear range of 0.08-1 ng/ml NA |
| Gene Symbol: |
Cd27 |
| Gene ID: |
21940 |
| Uniprot ID: |
CD27_MOUSE |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Mouse CD27 including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | In thymus and spleen, but not in non-lymphoid tissues. Expressed on myeloid progenitor cells. |
| Post Translational Modifications | Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. |
| Protein Name | Cd27 AntigenCd27l ReceptorT-Cell Activation Antigen Cd27Tumor Necrosis Factor Receptor Superfamily Member 7Cd Antigen Cd27 |
| Database Links | Reactome: R-MMU-5669034 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Cd27 Antigen proteinCd27l Receptor proteinT-Cell Activation Antigen Cd27 proteinTumor Necrosis Factor Receptor Superfamily Member 7 proteinCd Antigen Cd27 proteinCd27 proteinTnfrsf7 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
