Human TNFRSF7/CD27 protein (Recombinant) (hIgG-His-Tag) (STJP019492)
SPECIFICATIONS
HostHEK293 Cells
ImmunogenHuman
General Information
| Short Description | Recombinant-Human TNFRSF7/CD27-hIgG-His-Tag protein was developed from hek293 cells and has a target region of hIgG-His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Bioactivity |
| Host | HEK293 Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7. The ED50 range ≤ 0.8 ug/ml. |
Target Information
| Gene Symbol | CD27 |
| Gene ID | 939 |
| Uniprot ID | CD27_HUMAN |
| Accession Number | NP_001233.1 |
| Immunogen | Human |
| Immunogen Region | 20-191aa |
| Immunogen Sequence | ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IR |
Additional Info
| Tissue Specificity | Found in most T-lymphocytes. |
| Post Translational Modifications | Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. |
| Protein Name | Cd27 AntigenCd27l ReceptorT-Cell Activation Antigen Cd27T14Tumor Necrosis Factor Receptor Superfamily Member 7Cd Antigen Cd27 |
| Database Links | Reactome: R-HSA-5669034 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Cd27 Antigen proteinCd27l Receptor proteinT-Cell Activation Antigen Cd27 proteinT14 proteinTumor Necrosis Factor Receptor Superfamily Member 7 proteinCd Antigen Cd27 proteinCD27 proteinTNFRSF7 protein |
Information sourced from Uniprot.org