Mouse Ccl3 (C-C motif chemokine 3) Sandwich ELISA Kit (STJE0004207)

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STJE0004207
£163.50 - £357.50
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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description : This Ccl3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl3
Gene ID: 20302
Uniprot ID: CCL3_MOUSE
Specificity: This kit recognizes Mouse MIP-1 Alpha in samples. No significant cross-reactivity or interference between Mouse MIP-1 Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Protein Name C-C Motif Chemokine 3
Heparin-Binding Chemotaxis Protein
L2g25b
Macrophage Inflammatory Protein 1-Alpha
Mip-1-Alpha
Sis-Alpha
Small-Inducible Cytokine A3
Ty-5
Database Links Reactome: R-MMU-380108
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 3 ELISA kit
Heparin-Binding Chemotaxis Protein ELISA kit
L2g25b ELISA kit
Macrophage Inflammatory Protein 1-Alpha ELISA kit
Mip-1-Alpha ELISA kit
Sis-Alpha ELISA kit
Small-Inducible Cytokine A3 ELISA kit
Ty-5 ELISA kit
Ccl3 ELISA kit
Mip1a ELISA kit
Scya3 ELISA kit
Specificity This kit recognizes Mouse MIP-1α in samples. No significant cross-reactivity or interference between Mouse MIP-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 92
EDTA plasma (n=8) 90-103 95
Cell culture media (n=8) 89-105 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.90 154.80 386.60 42.70 160.00 368.20
Standard deviation 2.40 8.00 13.90 2.90 9.40 19.90
CV (%) 5.12 5.17 3.60 6.79 5.88 5.40

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