Mouse CCL2/MCP-1 protein (Recombinant) (No tag) (STJP002834)
SPECIFICATIONS
HostPichia
ConjugationUnconjugated
ImmunogenRecombinant Mouse CCL2/MCP-1 Protein is produced by Pichia expression system. The target protein is expressed with sequence (Gln 24-Asn148) of mouse CCL2/MCP-1 (Accession #NP_035463.1) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Mouse CCL2/MCP-1-No tag protein was developed in pichia using the region Gln 24-Asn148. For use in research applications. |
| Host | Pichia |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by the ability to inhibit the proliferation of HUVEC (Human Umbilical Vein Endothelial Cells).The ED50 for this effect is 0.20-0.80 ng/mL.|2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effec |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Ccl2 |
| Gene ID | 20296 |
| Uniprot ID | CCL2_MOUSE |
| Immunogen | Recombinant Mouse CCL2/MCP-1 Protein is produced by Pichia expression system. The target protein is expressed with sequence (Gln 24-Asn148) of mouse CCL2/MCP-1 (Accession #NP_035463.1) fused with no additional amino acid. |
| Immunogen Region | Gln 24-Asn148 |
| Immunogen Sequence | QPDAVNAPLTCCYSFTSKMI PMSRLESYKRITSSRCPKEA VVFVTKLKREVCADPKKEWV QTYIKNLDRNQMRSEPTTLF KTASALRSSAPLNVKLTRKS EANASTTFSTTTSSTSVGVT SVTVN |
Additional Info
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. |
| Protein Name | C-C Motif Chemokine 2Monocyte Chemoattractant Protein 1Monocyte Chemotactic Protein 1Mcp-1Platelet-Derived Growth Factor-Inducible Protein JeSmall-Inducible Cytokine A2 |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Protein Names | C-C Motif Chemokine 2 proteinMonocyte Chemoattractant Protein 1 proteinMonocyte Chemotactic Protein 1 proteinMcp-1 proteinPlatelet-Derived Growth Factor-Inducible Protein Je proteinSmall-Inducible Cytokine A2 proteinCcl2 proteinJe proteinMcp1 proteinScya2 protein |
Information sourced from Uniprot.org