| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Mouse Cathepsin B-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to cleave a flourogenic substrate, the measured activity is 2 nmole/ug/min NA |
| Gene Symbol: | Ctsb |
| Gene ID: | 13030 |
| Uniprot ID: | CATB_MOUSE |
| Immunogen Region: | HIS18-PHE339 |
| Immunogen: | Optimized DNA sequence encoding mature chain of mouse Cathepsin B (HIS18-PHE339) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in thyroid epithelial cells. |
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis. |
| Protein Name | Cathepsin B Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain |
| Database Links | Reactome: R-MMU-1442490 Reactome: -MMU-1679131 Reactome: -MMU-2022090 Reactome: -MMU-2132295 Reactome: -MMU-6798695 |
| Cellular Localisation | Lysosome Melanosome Secreted Extracellular Space Apical Cell Membrane Peripheral Membrane Protein Extracellular Side Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
| Alternative Protein Names | Cathepsin B protein Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain protein Ctsb protein |
Information sourced from Uniprot.org