| Applications: | CLIA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description : | This mouse AR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Areg |
| Gene ID: | 11839 |
| Uniprot ID: | AREG_MOUSE |
| Specificity: | This kit recognizes Mouse AR in samples. No significant cross-reactivity or interference between Mouse AR and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. |
| Protein Name | AmphiregulinArSchwannoma-Derived Growth FactorSdgf |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-177929Reactome: -MMU-179812Reactome: -MMU-180292Reactome: -MMU-180336Reactome: -MMU-182971Reactome: -MMU-204005Reactome: -MMU-212718Reactome: -MMU-5673001Reactome: -MMU-5694530Reactome: -MMU-6811558Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-9009391 |
| Cellular Localisation | MembraneSingle-Pass Membrane Protein |
| Alternative CLIA Names | Amphiregulin CLIA kitAr CLIA kitSchwannoma-Derived Growth Factor CLIA kitSdgf CLIA kitAreg CLIA kitSdgf CLIA kit |
| Specificity | This kit recognizes Mouse AR in samples. No significant cross-reactivity or interference between Mouse AR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-105 | 98 |
| EDTA plasma(n=8) | 96-109 | 102 |
| Cell culture media(n=8) | 90-104 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 25.70 | 64.64 | 182.33 | 25.19 | 65.30 | 194.06 |
| Standard deviation | 2.21 | 7.41 | 16.59 | 2.75 | 6.28 | 17.39 |
| CV (%) | 8.60 | 11.46 | 9.10 | 10.92 | 9.62 | 8.96 |
