| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Mouse alpha 1 antitrypsin-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRKDnp-NH2, with a calculated ED50 < 4.5nM NA |
| Gene Symbol: | Serpina1a |
| Gene ID: | 20700 |
| Uniprot ID: | A1AT1_MOUSE |
| Immunogen Region: | GLU25-LYS413 |
| Immunogen: | Optimized DNA sequence encoding mature chain of Mouse alpha 1 antitrypsin-A1AT (GLU25-LYS413) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. |
| Protein Name | Alpha-1-Antitrypsin 1-1AatAlpha-1 Protease Inhibitor 1Alpha-1-AntiproteinaseSerine Protease Inhibitor 1-1Serine Protease Inhibitor A1aSerpin A1a |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Alpha-1-Antitrypsin 1-1 proteinAat proteinAlpha-1 Protease Inhibitor 1 proteinAlpha-1-Antiproteinase proteinSerine Protease Inhibitor 1-1 proteinSerine Protease Inhibitor A1a proteinSerpin A1a proteinSerpina1a proteinDom1 proteinSpi1-1 protein |
Information sourced from Uniprot.org
