Human VHL protein (Recombinant) (His-Tag) (STJP019804)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human VHL-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in phosphate-buffered Saline (pH 7.4) containing 1mM DTT, 2mM EDTA |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VHL |
| Gene ID | 7428 |
| Uniprot ID | VHL_HUMAN |
| Accession Number | NP_000542.1 |
| Immunogen | Human |
| Immunogen Region | 1-154aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in the adult and fetal brain and kidney. |
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor SuppressorProtein G7Pvhl |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-3232142Reactome: R-HSA-8951664Reactome: R-HSA-9682706Reactome: R-HSA-9694686Reactome: R-HSA-9706019Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic ReticulumNucleusFound Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-AssociatedColocalizes With Adrb2 At The Cell MembraneIsoform 3: CytoplasmEqually Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Protein Names | Von Hippel-Lindau Disease Tumor Suppressor proteinProtein G7 proteinPvhl proteinVHL protein |
Information sourced from Uniprot.org