| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Siglec-3/CD33-C-hFc&His protein was developed from hek293 cells and has a target region of C-hFc&His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human CD33 Protein at 1 Mu g/mL (100 Mu L/well) can bind CD33 Rabbit pAb with a linear range of 0.03-1.66 ng/mL. |
| Gene Symbol: |
CD33 |
| Gene ID: |
945 |
| Uniprot ID: |
CD33_HUMAN |
| Immunogen: |
Recombinant Human Siglec-3/CD33 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asp18-His259) of human CD33/Siglec-3 (Accession #NP_001763.3) fused with an Fc, 6×His tag at the C-terminus. |
| Tissue Specificity | Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells. |
| Post Translational Modifications | Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition. Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent. |
| Function | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K. |
| Protein Name | Myeloid Cell Surface Antigen Cd33Sialic Acid-Binding Ig-Like Lectin 3Siglec-3Gp67Cd Antigen Cd33 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform Cd33m: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Cd33m: PeroxisomeCd33m Isoform Does Not Localize To Cell Surfaces But Instead Accumulates In Peroxisomes |
| Alternative Protein Names | Myeloid Cell Surface Antigen Cd33 proteinSialic Acid-Binding Ig-Like Lectin 3 proteinSiglec-3 proteinGp67 proteinCd Antigen Cd33 proteinCD33 proteinSIGLEC3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
