• Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)
  • Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)
  • Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)
  • Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)
  • Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)
  • Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)

Human PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP000843)

SKU:
STJP000843

Current Stock:
Host: HEK293 cells
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human PD-1/PDCD1/CD279-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications.
Formulation: Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation.
Immunoreactivity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human PD-1 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human PD-L1 with a linear range of 0.5-2 Mu g/mL.
Gene Symbol: PDCD1
Gene ID: 5133
Uniprot ID: PDCD1_HUMAN
Immunogen: Recombinant Human PD-1/PDCD1/CD279 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Thr168) of human PD-1 (Accession #NP_005009.2) fused with a 6×His tag at the C-terminus.
Post Translational Modifications Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. N-glycosylation at Asn-58 contains at least two N-acetylglucosamine units and one fucose. N-glycosylation does not affect binding to nivolumab drug.
Function Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name Programmed Cell Death Protein 1
Protein Pd-1
Hpd-1
Cd Antigen Cd279
Database Links Reactome: R-HSA-389948
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Programmed Cell Death Protein 1 protein
Protein Pd-1 protein
Hpd-1 protein
Cd Antigen Cd279 protein
PDCD1 protein
PD1 protein

Information sourced from Uniprot.org

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