Mouse PD-1/PDCD1/CD279 protein (Recombinant) (C-His) (STJP001181)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse PD-1/PDCD1/CD279 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Gln167) of mouse PD-1 (Accession #NP_032824.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse PD-1/PDCD1/CD279-C-His protein was developed in hek293 cells using the region Leu25-Gln167. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Pdcd1 |
| Gene ID | 18566 |
| Uniprot ID | PDCD1_MOUSE |
| Immunogen | Recombinant Mouse PD-1/PDCD1/CD279 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Gln167) of mouse PD-1 (Accession #NP_032824.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Leu25-Gln167 |
| Immunogen Sequence | LEVPNGPWRSLTFYPAWLTV SEGANATFTCSLSNWSEDLM LNWNRLSPSNQTEKQAAFCN GLSQPVQDARFQIIQLPNRH DFHMNILDTRRNDSGIYLCG AISLHPKAKIEESPGAELVV TERILETSTRYPSPSPKPEG RFQ |
Additional Info
| Post Translational Modifications | Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Deubiquitinated and thus stabilized by USP5. Tyrosine phosphorylated at Tyr-225 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. Phosphorylation at Thr-236 promotes the recruitment of the deubiquitinase USP5. |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Mpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Programmed Cell Death Protein 1 proteinProtein Pd-1 proteinMpd-1 proteinCd Antigen Cd279 proteinPdcd1 proteinPd1 protein |
Information sourced from Uniprot.org