| Post Translational Modifications | There appear to be no free sulfhydryl groups. |
| Function | Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. |
| Protein Name | Pappalysin-1Insulin-Like Growth Factor-Dependent Igf-Binding Protein 4 ProteaseIgf-Dependent Igfbp-4 ProteaseIgfbp-4asePregnancy-Associated Plasma Protein APapp-A |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Pappalysin-1 ELISA kitInsulin-Like Growth Factor-Dependent Igf-Binding Protein 4 Protease ELISA kitIgf-Dependent Igfbp-4 Protease ELISA kitIgfbp-4ase ELISA kitPregnancy-Associated Plasma Protein A ELISA kitPapp-A ELISA kitPAPPA ELISA kit |
| Specificity | This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 91 |
| EDTA plasma (n=8) | 95-106 | 100 |
| Cell culture media (n=8) | 88-98 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.70 | 6.80 | 17.60 | 2.50 | 6.80 | 17.30 |
| Standard deviation | 0.10 | 0.40 | 0.70 | 0.10 | 0.30 | 0.80 |
| CV (%) | 3.70 | 5.88 | 3.98 | 4.00 | 4.41 | 4.62 |
