| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human SPP1 Protein at 1 Mu g/mL (100 Mu L/well) can bind Osteopontin Rabbit mAb with a linear range of 0.976-11.58 ng/mL. |
| Gene Symbol: | SPP1 |
| Gene ID: | 6696 |
| Uniprot ID: | OSTP_HUMAN |
| Immunogen Region: | Ile17-Asn300 |
| Immunogen: | Recombinant Human Osteopontin/SPP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_000573.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | IPVKQADSGSSEEKQLYNKY PDAVATWLNPDPSQKQNLLA PQTLPSKSNESHDHMDDMDD EDDDDHVDSQDSIDSNDSDD VDDTDDSHQSDESHHSDESD ELVTDFPTDLPATEVFTPVV PTVDTYDGRGDSVVYGLRSK SKKFRRPDIQYPDATDEDIT SHMESEELNGAYKAIPVAQD LNAPSDWDSRGKDSYETSQL DDQSAETHSHKQSRLYKRKA NDESNEHSDVIDSQELSKV |
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275Reactome: R-HSA-9856532 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Osteopontin proteinBone Sialoprotein 1 proteinNephropontin proteinSecreted Phosphoprotein 1 proteinSpp-1 proteinUrinary Stone Protein proteinUropontin proteinSPP1 proteinBNSP proteinOPN proteinPSEC0156 protein |
Information sourced from Uniprot.org
