• Biotinylated Human Notch 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
  • The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.

Human Notch 1 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001651)

SKU:
STJP001651

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Notch 1-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: NOTCH1
Gene ID: 4851
Uniprot ID: NOTC1_HUMAN
Immunogen Region: Ala19-Gln526
Immunogen: DNA sequence encoding Human Notch 1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by one of the catalytic subunits of gamma-secretase (PSEN1 or PSEN2), to release a Notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. O-glycosylated on the EGF-like domains. O-glucosylated at Ser-435 by KDELC1 and KDELC2. Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4. O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). MFNG-, RFNG- and LFNG-mediated modification of O-fucose residues at specific EGF-like domains results in inhibition of its activation by JAG1 and enhancement of its activation by DLL1 via an increased binding to DLL1. Ubiquitinated. Undergoes 'Lys-29'-linked polyubiquitination by ITCH.promotes the lysosomal degradation of non-activated internalized NOTCH1. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch. Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN. Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD.
Function Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis.negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Protein Name Neurogenic Locus Notch Homolog Protein 1
Notch 1
Hn1
Translocation-Associated Notch Protein Tan-1 Cleaved Into - Notch 1 Extracellular Truncation
Next - Notch 1 Intracellular Domain
Nicd
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2644607
Reactome: R-HSA-2660826
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-8941856
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Reactome: R-HSA-9793380
Reactome: R-HSA-9818749
Reactome: R-HSA-9824272
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 1 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Nuclear Location May Require Megf10
Alternative Protein Names Neurogenic Locus Notch Homolog Protein 1 protein
Notch 1 protein
Hn1 protein
Translocation-Associated Notch Protein Tan-1 Cleaved Into - Notch 1 Extracellular Truncation protein
Next - Notch 1 Intracellular Domain protein
Nicd protein
NOTCH1 protein
TAN1 protein

Information sourced from Uniprot.org

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