| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Nectin-3/CD113-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: |
Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: |
The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: |
NECTIN3 |
| Gene ID: |
25945 |
| Uniprot ID: |
NECT3_HUMAN |
| Immunogen Region: |
Gly58-Asp400 |
| Immunogen: |
DNA sequence encoding Human Nectin-3/CD113 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Function | Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types. Plays a role in the morphology of the ciliary body. |
| Protein Name | Nectin-3Cdw113Nectin Cell Adhesion Molecule 3Poliovirus Receptor-Related Protein 3Cd Antigen Cd113 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-420597 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinPostsynaptic Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionAdherens JunctionIn The Auditory EpitheliumSpecificaly Localizes To The Apical Side Of The Lateral Membranes Of Supporting Cells |
| Alternative Protein Names | Nectin-3 proteinCdw113 proteinNectin Cell Adhesion Molecule 3 proteinPoliovirus Receptor-Related Protein 3 proteinCd Antigen Cd113 proteinNECTIN3 proteinPRR3 proteinPVRL3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
