| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | 1.Measured in a cell migration assay using A549 cells. 1 ng/mL of Recombinant Human MMP-1 can effectively induce A549 cells migration.|2.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity i |
| Gene Symbol: | MMP1 |
| Gene ID: | 4312 |
| Uniprot ID: | MMP1_HUMAN |
| Immunogen Region: | Phe20-Asn469 |
| Immunogen: | Recombinant Human MMP1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe20-Asn469) of human MMP1 (Accession #NP_002412.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | FPATLETQEQDVDLVQKYLE KYYNLKNDGRQVEKRRNSGP VVEKLKQMQEFFGLKVTGKP DAETLKVMKQPRCGVPDVAQ FVLTEGNPRWEQTHLTYRIE NYTPDLPRADVDHAIEKAFQ LWSNVTPLTFTKVSEGQADI MISFVRGDHRDNSPFDGPGG NLAHAFQPGPGIGGDAHFDE DERWTNNFREYNLHRVAAHE LGHSLGLSHSTDIGALMYPS YTFSGDVQLAQDDIDGIQA |
| Post Translational Modifications | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. |
| Function | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. |
| Protein Name | Interstitial Collagenase Fibroblast Collagenase Matrix Metalloproteinase-1 Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase |
| Database Links | Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389 Reactome: R-HSA-210991 Reactome: R-HSA-381426 Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted Extracellular Space Extracellular Matrix |
| Alternative Protein Names | Interstitial Collagenase protein Fibroblast Collagenase protein Matrix Metalloproteinase-1 protein Mmp-1 Cleaved Into - 22 Kda Interstitial Collagenase - 27 Kda Interstitial Collagenase protein MMP1 protein CLG protein |
Information sourced from Uniprot.org