| Host: | Mammalian Cells |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Active-Human MEPE/OF45-C-10*His protein was developed from mammalian cells and has a target region of C-10*His. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity: | Measured by its ability to induce adhesion of mouse chondrogenic cells (ATDC5). rhMEPE/OF45 Immobilised at 2 µg/mL (100 µL/well) , can induce more than 60% of ATDC5 cell adhesion. |
| Gene Symbol: | MEPE |
| Gene ID: | 56955 |
| Uniprot ID: | MEPE_HUMAN |
| Immunogen Region: | Pro18-Asp525 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Phosphorylated on serine residues in the ASARM motif (in vitro) by FAM20C.the phosphorylation is important for the inhibition of bone mineralization. Cleaved by CTSB/cathepsin B.the cleavage is blocked by metalloprotease PHEX. |
| Function | Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage. Promotes dental pulp stem cell proliferation and differentiation. |
| Protein Name | Matrix Extracellular PhosphoglycoproteinOsteoblast/Osteocyte Factor 45Of45Osteoregulin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Matrix Extracellular PhosphoglycoproteinOsteoblast/Osteocyte Factor 45 proteinOf45 proteinOsteoregulin proteinMEPE protein |
Information sourced from Uniprot.org