Human Malate dehydrogenase 2/MDH2 protein (Recombinant) (His-Tag) (STJP016819)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP016819
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General Information

Short DescriptionRecombinant-Human Malate dehydrogenase 2/MDH2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Enzyme Activity
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 7.5) containing 10% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin< 1 EU per 1ug of protein (determined by LAL method)
ImmunoreactivitySpecific activity is > 700unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Target Information

Gene SymbolMDH2
Gene ID4191
Uniprot IDMDHM_HUMAN
Accession NumberNP_005909
ImmunogenHuman
Immunogen Region25-338aa
Immunogen SequenceAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGR

Additional Info

Post Translational Modifications Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.
Protein Name Malate Dehydrogenase - Mitochondrial
Database Links Reactome: R-HSA-71403
Reactome: R-HSA-9837999
Reactome: R-HSA-9856872
Cellular Localisation Mitochondrion Matrix
Alternative Protein Names Malate Dehydrogenase - Mitochondrial protein
MDH2 protein

Information sourced from Uniprot.org

Citations

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