| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | SFTPD |
| Gene ID: | 6441 |
| Uniprot ID: | SFTPD_HUMAN |
| Immunogen: | Recombinant Human Lung surfactant protein D/SFTPD (E22G) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala21-Phe375 (Glu22Gly) ) of human SFTPD/SP-D/SFTP4 (Accession #NP_003010.4) fused with a 6×His t |
| Immunogen Sequence: | AEMKTYSHRTMPSACTLVMC SSVESGLPGRDGRDGREGPR GEKGDPGLPGAAGQAGMPGQ AGPVGPKGDNGSVGEPGPKG DTGPSGPPGPPGVPGPAGRE GPLGKQGNIGPQGKPGPKGE AGPKGEVGAPGMQGSAGARG LAGPKGERGVPGERGVPGNT GAAGSAGAMGPQGSPGARGP PGLKGDKGIPGDKGAKGESG LPDVASLRQQVEALQGQVQH LQAAFSQYKKVELFPNGQS |
| Tissue Specificity | Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). |
| Post Translational Modifications | The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages. |
| Function | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
| Protein Name | Pulmonary Surfactant-Associated Protein DPsp-DSp-DCollectin-7Lung Surfactant Protein D |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-168179Reactome: R-HSA-198933Reactome: R-HSA-391160Reactome: R-HSA-5683826Reactome: R-HSA-5686938Reactome: R-HSA-5688849Reactome: R-HSA-5688890Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixSurface Film |
| Alternative Protein Names | Pulmonary Surfactant-Associated Protein D proteinPsp-D proteinSp-D proteinCollectin-7 proteinLung Surfactant Protein D proteinSFTPD proteinCOLEC7 proteinPSPD proteinSFTP4 protein |
Information sourced from Uniprot.org