| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of 50mM NaAc, 150mM NaCl, 10% Glycerol (pH 5.0) |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
<1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: |
PLA2G7 |
| Gene ID: |
7941 |
| Uniprot ID: |
PAFA_HUMAN |
| Immunogen Region: |
Phe22-Asn441 |
| Immunogen: |
Recombinant Human Lp-PLA2/PLA2G7 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe22-Asn441) of human Lp-PLA2/PLA2G7 (Accession #NP_001161829.1) fused with a His tag at the C-terminus. |
| Immunogen Sequence: |
EEELRSKSKICANVFCGAGR ECAVTEKGEPTCLCIEQCKP HKRPVCGSNGKTYLNHCELH RDACLTGSKIQVDYDGHCKE KKSVSPSASPVVCYQSNRDE LRRRIIQWLEAEIIPDGWFS KGSNYSEILDKYFKNFDNGD SRLDSSEFLKFVEQNETAIN ITTYPDQENNKLLRGLCVDA LIELSDENADWKLSFQEFLK CLNPSFNPPEKKCALEDETY ADGAETEVDCNRCVCACGN |
| Tissue Specificity | Plasma. Secreted by macrophages (at protein level). |
| Post Translational Modifications | N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles. |
| Function | Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage. |
| Protein Name | Platelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase1-Alkyl-2-Acetylglycerophosphocholine Esterase2-Acetyl-1-Alkylglycerophosphocholine EsteraseGroup-Viia Phospholipase A2Gviia-Pla2Ldl-Associated Phospholipase A2Ldl-Pla(2Paf 2-Acylhydrolase |
| Database Links | Reactome: R-HSA-422085 |
| Cellular Localisation | SecretedExtracellular SpaceAssociates With Both Ldl And Hdl Particles In PlasmaMainly Associates With Pro-Inflammatory Electronegative Ldl Particles |
| Alternative Protein Names | Platelet-Activating Factor Acetylhydrolase proteinPaf Acetylhydrolase protein1-Alkyl-2-Acetylglycerophosphocholine Esterase protein2-Acetyl-1-Alkylglycerophosphocholine Esterase proteinGroup-Viia Phospholipase A2 proteinGviia-Pla2 proteinLdl-Associated Phospholipase A2 proteinLdl-Pla(2 proteinPaf 2-Acylhydrolase proteinPLA2G7 proteinPAFAH protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
