Human LILRB1/CD85j/ILT2 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001602)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding Human LILRB1/CD85j/ILT2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
General Information
| Short Description | Recombinant-Human LILRB1/CD85j/ILT2-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Formulation | Lyophilised from 0.2 ยตm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20ยฐC. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). |
Target Information
| Gene Symbol | LILRB1 |
| Gene ID | 10859 |
| Uniprot ID | LIRB1_HUMAN |
| Immunogen | DNA sequence encoding Human LILRB1/CD85j/ILT2 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Immunogen Region | Gly24-His458 |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. |
| Function | Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of FCER1A signaling and serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide. Upon interaction with peptide-bound HLA-G-B2M complex, triggers secretion of growth-promoting factors by decidual NK cells. Reprograms B cells toward an immune suppressive phenotype. |
| Protein Name | Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 1Lir-1Leukocyte Immunoglobulin-Like Receptor 1Cd85 Antigen-Like Family Member JImmunoglobulin-Like Transcript 2Ilt-2Monocyte/Macrophage Immunoglobulin-Like Receptor 7Mir-7Cd Antigen Cd85j |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 5: Secreted |
| Alternative Protein Names | Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 1 proteinLir-1 proteinLeukocyte Immunoglobulin-Like Receptor 1 proteinCd85 Antigen-Like Family Member J proteinImmunoglobulin-Like Transcript 2 proteinIlt-2 proteinMonocyte/Macrophage Immunoglobulin-Like Receptor 7 proteinMir-7 proteinCd Antigen Cd85j proteinLILRB1 proteinILT2 proteinLIR1 proteinMIR7 protein |
Information sourced from Uniprot.org