Name: Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA Kit (STJE0003429)
SKU: STJE0003429
Availability: InStock

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	3.75mIU/mL
Detection Limit:	6.25~400mIU/mL
Short Description :	This KRT18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	KRT18
Gene ID:	3875
Uniprot ID:	K1C18_HUMAN
Specificity:	This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function	Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name	Keratin - Type I Cytoskeletal 18 Cell Proliferation-Inducing Gene 46 Protein Cytokeratin-18 Ck-18 Keratin-18 K18
Database Links	Reactome: R-HSA-6805567 Reactome: R-HSA-6809371
Cellular Localisation	Nucleus Matrix Cytoplasm Perinuclear Region Nucleus Nucleolus
Alternative ELISA Names	Keratin - Type I Cytoskeletal 18 ELISA kit Cell Proliferation-Inducing Gene 46 Protein ELISA kit Cytokeratin-18 ELISA kit Ck-18 ELISA kit Keratin-18 ELISA kit K18 ELISA kit KRT18 ELISA kit CYK18 ELISA kit PIG46 ELISA kit
Specificity	This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (mIU/mL)	18.51	61.86	159.46	19.61	63.54	150.53
Standard deviation	1.12	2.50	7.35	1.13	3.18	5.81
CV (%)	6.05	4.04	4.61	5.76	5.00	3.86

Model	Name	Host	Clonality	Reactivity	Application
STJE0010224	Mouse KRT18 (Keratin 18) Sandwich ELISA Kit Kit (STJE0010224)			Mouse	ELISA
STJE0010225	Porcine KRT18 (Keratin 18) Sandwich ELISA Kit Kit (STJE0010225)			Porcine	ELISA

Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA Kit (STJE0003429)