| Host: | CHO cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Interleukin-10-protein was developed from cho cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution pH7.0. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED50 determined by dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be ≤ ng/ml, corresponding to a specific activity of ≥x units/mg. NA |
| Gene Symbol: | IL10 |
| Gene ID: | 3586 |
| Uniprot ID: | IL10_HUMAN |
| Immunogen Region: | Mature chain |
| Immunogen: | Optimized DNA sequence encoding Human Interleukin-10 mature chain was expressed in CHO Cell NA |
| Immunogen Sequence: | MHSSALLCCL VLLTGVRASP GQGTQSENSC THFPGNLPNM LRDLRDAFSR VKTFFQMKDQ LDNLLLKESL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN QDPDIKAHVN SLGENLKTLR LRLRRCHRFL PCENKSKAVE QVKNAFNKLQ EKGIYKAMSE FDIFINYIEA YMTMKIRN NA |
| Tissue Specificity | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10 Il-10 Cytokine Synthesis Inhibitory Factor Csif |
| Database Links | Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-8950505 Reactome: R-HSA-9662834 Reactome: R-HSA-9664323 Reactome: R-HSA-9725370 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-10 protein Il-10 protein Cytokine Synthesis Inhibitory Factor protein Csif protein IL10 protein |
Information sourced from Uniprot.org