• Following the initial culture period, islets were cultured for an additional 6–24 hours in CMRL 1066 containing antibiotics, 2 mM glutamine and one of the following supplements: 0. 5 mM sodium palmitate solubilized in 0. 5% (weight/volume) fatty acid and lipopolysaccaride free bovine serum albumin (BSA) ; recombinant human Interleukin 1beta (IL-1ß (50 units/ml) and Interferon-gamma (IFN-Gamma ) (1,000 units/ml) ; 100 mM hydrogen peroxide; 2 mM DETA/NO or 10 mM streptozotocin (STZ). To some of the groups 10 µM of Imatinib was added at different time points prior to the addition of test substances given above. To controls equal amounts of vehicle (DMSO) were supplemented.
  • Interferon gamma (IFN-Gamma ) 50 Mu l (100 ng/ml) was added to each dish in the experimental studies. The cytoplasmic and nuclear extracts were washed with ice-cold PBS and lysed in a 0. 5 ml/well lysis buffer (150 mmol/l NaCl, 20 mmol/l Tris, pH 7. 5,0. 1% Triton X-100, 1 mmol/l phenylmethylsulfonyl fluoride [PMSF] and 10 Mu g/ml aprotonin) as modified from the reports of Kim et al. and Moon et al. [
  • E) Recombinant Type III Interferons in the absence of CM at the same concentrations as found in the CM (IL-28A/IFN Lambda 2: 1500 pg/mL; IL-28B/IFN Lambda 3: 10 pg/mL; IL-29/IFN Lambda 1: 500 pg/mL) were added to JFH-1 infected Huh7. 5. 1 cells.
  • Venous blood was collected prior to chemotherapy or at least one month following chemotherapy. For the offspring (HLA haploidentical donors) , the routine blood tests, and liver and kidney function tests were normal and negative for hepatitis A virus-IgM, hepatitis B surface antigen antibody, hepatitis B e--hepatitis B core-hepatitis C virus (HCV) -HCV-IgG, Syphilis-and human immunodeficiency virus-. As reported in our previous study (2 atmosphere. On the day of culture, 1,000 U/ml human recombinant interferon-Gamma, 500 U/ml recombinant human interleukin (rhIL) -1 Alpha and 1,000 U/ml rhIL-2 (Quangang Pharmaceutical Co. , , , ) were added. Four days later, 1,000 U/ml rhIL-2 was added and the cells were transferred into a GT-T610 culture bag. Cell growth was observed every other day and cells were stained with 0. 4% trypan blue and the viable cells were counted. Following 18 days of culture, cells were infused once daily (>1×109 cells; viability rate, >95%). A cycle of treatment…

Human Interferon-beta 1b protein (Recombinant) (STJP000324)

SKU:
STJP000324

£136.50 - £228.50
Current Stock:
Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Interferon-beta 1b-protein was developed from cho cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered solution containing mM NaOAc pH.5.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: Theactivity was determined by the cytopathic inhibition assay of human WISH cells infected with the ECMV virus, and determined to bex107 IU/mg. NA
Gene Symbol: IFNB1
Gene ID: 3456
Uniprot ID: IFNB_HUMAN
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Human beta Interferon 1b mature chain was expressed in Chinese Hamster Ovary cells. NA
Immunogen Sequence: MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN NA
Function Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways. Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor. Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth.
Protein Name Interferon Beta
Ifn-Beta
Fibroblast Interferon
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-918233
Reactome: R-HSA-933541
Reactome: R-HSA-9705671
Reactome: R-HSA-983231
Cellular Localisation Secreted
Alternative Protein Names Interferon Beta protein
Ifn-Beta protein
Fibroblast Interferon protein
IFNB1 protein
IFB protein
IFNB protein

Information sourced from Uniprot.org

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