| Host: |
E.coli |
| Applications: |
SDS-PAGE |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Recombinant-Human Importin beta/KPNB1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: |
Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% Glycerol, 0.1M NaCl |
| Concentration: |
0.25 mg/mL |
| Storage Instruction: |
For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KPNB1 |
| Gene ID: |
3837 |
| Uniprot ID: |
IMB1_HUMAN |
| Immunogen Region: |
1-876aa |
| Accession Number: |
NP_002256.2 |
| Immunogen: |
Human |
| Immunogen Sequence: |
MELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLE |
| Post Translational Modifications | Mono-ADP-ribosylated by PARP16. |
| Function | Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports MRTFA, SNAI1 and PRKCI into the nucleus. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. |
| Protein Name | Importin Subunit Beta-1Importin-90Karyopherin Subunit Beta-1Nuclear Factor P97Pore Targeting Complex 97 Kda SubunitPtac97 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-140342Reactome: R-HSA-1655829Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-180746Reactome: R-HSA-2995383Reactome: R-HSA-6798695Reactome: R-HSA-68616Reactome: R-HSA-909733Reactome: R-HSA-9615933Reactome: R-HSA-9636249Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmNucleus Envelope |
| Alternative Protein Names | Importin Subunit Beta-1 proteinImportin-90 proteinKaryopherin Subunit Beta-1 proteinNuclear Factor P97 proteinPore Targeting Complex 97 Kda Subunit proteinPtac97 proteinKPNB1 proteinNTF97 protein |
Information sourced from Uniprot.org
