| Host: |
E. coli |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its ability to enhance Bcl2 expression in HCT116 human colon adenocarcinoma cells. 0.01-1ng/mL of Recombinant Human IL-8 can effectively enhance Bcl2 expression. |
| Gene Symbol: |
CXCL8 |
| Gene ID: |
3576 |
| Uniprot ID: |
IL8_HUMAN |
| Immunogen: |
Recombinant Human IL-8/CXCL8/GCP-1 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala23-Ser99) of human IL-8 (Accession #NP_000575.1). |
| Immunogen Sequence: |
AVLPRSAKELRCQCIKTYSK PFHPKFIKELRVIESGPHCA NTEIIVKLSDGRELCLDPKE NWVQRVVEKFLKRAENS |
| Post Translational Modifications | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP.leading to impaired neutrophil endothelial transmigration and thus increased virulence. |
| Function | Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. |
| Protein Name | Interleukin-8Il-8C-X-C Motif Chemokine 8ChemokineC-X-C Motif Ligand 8EmoctakinGranulocyte Chemotactic Protein 1Gcp-1Monocyte-Derived Neutrophil Chemotactic FactorMdncfMonocyte-Derived Neutrophil-Activating PeptideMonapNeutrophil-Activating Protein 1Nap-1Protein 3-10cT-Cell Chemotactic Factor Cleaved Into - Mdncf-AGcp/Il-8 Protein IvIl8/Nap1 Form I - Interleukin-8(Ala-Il-877Gcp/Il-8 Protein IiIl-8(1-77Il8/Nap1 Form IiMdncf-B - Il-8(5-77 - Il-8(6-77(Ser-Il-872Gcp/Il-8 Protein IIl8/Nap1 Form IiiLymphocyte-Derived Neutrophil-Activating FactorLynapMdncf-CNeutrophil-Activating FactorNaf - Il-8(7-77Gcp/Il-8 Protein VIl8/Nap1 Form Iv - Il-8(8-77Gcp/Il-8 Protein ViIl8/Nap1 Form V - Il-8(9-77Gcp/Il-8 Protein IiiIl8/Nap1 Form Vi |
| Database Links | Reactome: R-HSA-2559582Reactome: R-HSA-375276Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-418594Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-8 proteinIl-8 proteinC-X-C Motif Chemokine 8 proteinChemokine proteinC-X-C Motif Ligand 8 proteinEmoctakin proteinGranulocyte Chemotactic Protein 1 proteinGcp-1 proteinMonocyte-Derived Neutrophil Chemotactic Factor proteinMdncf proteinMonocyte-Derived Neutrophil-Activating Peptide proteinMonap proteinNeutrophil-Activating Protein 1 proteinNap-1 proteinProtein 3-10c proteinT-Cell Chemotactic Factor Cleaved Into - Mdncf-A proteinGcp/Il-8 Protein Iv proteinIl8/Nap1 Form I - Interleukin-8 protein(Ala-Il-877 proteinGcp/Il-8 Protein Ii proteinIl-8(1-77 proteinIl8/Nap1 Form Ii proteinMdncf-B - Il-8(5-77 - Il-8(6-77 protein(Ser-Il-872 proteinGcp/Il-8 Protein I proteinIl8/Nap1 Form Iii proteinLymphocyte-Derived Neutrophil-Activating Factor proteinLynap proteinMdncf-C proteinNeutrophil-Activating Factor proteinNaf - Il-8(7-77 proteinGcp/Il-8 Protein V proteinIl8/Nap1 Form Iv - Il-8(8-77 proteinGcp/Il-8 Protein Vi proteinIl8/Nap1 Form V - Il-8(9-77 proteinGcp/Il-8 Protein Iii proteinIl8/Nap1 Form Vi proteinCXCL8 proteinIL8 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
