| Short Description : | Recombinant-Human IL-3RA/CD123-C-His protein was developed in hek293 cells using the region Lys20-Arg305. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL3 at 4 Mu g/mL (100 Mu L/well) can bind recombinant Human IL3RA, the EC50 of Human IL3RA is 5.3 Mu g/mL. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | IL3RA |
| Gene ID: | 3563 |
| Uniprot ID: | IL3RA_HUMAN |
| Immunogen: | Recombinant Human IL-3RA/CD123 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys20-Arg305) of human IL3RA/CD123 (Accession #NP_002174.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region: | Lys20-Arg305 |
| Immunogen Sequence: | KEDPNPPITNLRMKAKAQQL TWDLNRNVTDIECVKDADYS MPAVNNSYCQFGAISLCEVT NYTVRVANPPFSTWILFPEN SGKPWAGAENLTCWIHDVDF LSCSWAVGPGAPADVQYDLY LNVANRRQQYECLHYKTDAQ GTRIGCRFDDISRLSSGSQS SHILVRGRSAAFGIPCTDKF VVFSQIEILTPPNMTAKCNK THSFMHWKMRSHFNRKFRYE LQIQKRMQPVITEQVRDRT |
| Post Translational Modifications | Ubiquitinated by RNFT2 in response to IL3. Ubiquitination leads ligand-induced degradation by the proteasome. Ubiquitinated by RNF128 via 'Lys-27'-linked polyubiquitination, facilitating its degradation through the lysosomal pathway. |
| Function | Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program. |
| Protein Name | Interleukin-3 Receptor Subunit AlphaIl-3 Receptor Subunit AlphaIl-3r Subunit AlphaIl-3r-AlphaIl-3raCd Antigen Cd123 |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-912526 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Interleukin-3 Receptor Subunit Alpha proteinIl-3 Receptor Subunit Alpha proteinIl-3r Subunit Alpha proteinIl-3r-Alpha proteinIl-3ra proteinCd Antigen Cd123 proteinIL3RA proteinIL3R protein |
Information sourced from Uniprot.org
