| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Active Recombinant-Human IL-3-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Immunoreactivity: |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.5-6 ng/mL.2. Recombinant Human TPO (50 ng/mL) , IL-3 (15 ng/mL) , IL-6 (15 ng/mL) and IL-11 (15 ng/mL) induce hematopoietic stem and progenit |
| Gene Symbol: |
IL3 |
| Gene ID: |
3562 |
| Uniprot ID: |
IL3_HUMAN |
| Immunogen: |
Recombinant Human IL-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala20-Phe152) of human interleukin-3/IL-3 (Accession #NP_000579.2) fused with a 6×His tag at the C-terminus. |
| Function | Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB. Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program. Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK. |
| Protein Name | Interleukin-3Il-3Hematopoietic Growth FactorMast Cell Growth FactorMcgfMultipotential Colony-Stimulating FactorP-Cell-Stimulating Factor |
| Database Links | Reactome: R-HSA-512988Reactome: R-HSA-5673001Reactome: R-HSA-8939247Reactome: R-HSA-912526 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-3 proteinIl-3 proteinHematopoietic Growth Factor proteinMast Cell Growth Factor proteinMcgf proteinMultipotential Colony-Stimulating Factor proteinP-Cell-Stimulating Factor proteinIL3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
