Human IgM protein (Recombinant) (C-His) (STJP003087)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IgM Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln218-Tyr453) of Human IgM (Accession #P01871-1) fused with a His tag at the N-terminus.
General Information
| Short Description | Recombinant-Human IgM-C-His protein was developed in hek293 cells using the region Gln218-Tyr453. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | <0.01 EU/Mu g |
Target Information
| Immunogen | Recombinant Human IgM Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln218-Tyr453) of Human IgM (Accession #P01871-1) fused with a His tag at the N-terminus. |
| Immunogen Region | Gln218-Tyr453 |
| Immunogen Sequence | MIIVAHVLLILLGATEILQA DLLPDEKISLLPPVNFTIKV TGLAQVLLQWKPNPDQEQRN VNLEYQVKINAPKEDDYETR ITESKCVTILHKGFSASVRT ILQNDHSLLASSWASAELHA PPGSPGTSIVNLTCTTNTTE DNYSRLRSYQVSLHCTWLVG TDAPEDTQYFLYYRYGSWTE ECQEYSKDTLGRNIACWFPR TFILSKGRDWLAVLVNGSSK HSAIRPFDQLFALHAIDQI |
Additional Info
| Background | IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as natural blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt s lymphoma bear surface IgM, whereas plasmocytes from WaldenstrΓΆm s disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated. |
Information sourced from Uniprot.org