| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
<1 EU/Mu g |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human IGFBP3 Protein at 1 Mu g/mL (100 Mu L/well) can bind IGF1 with a linear range of 0.158-23.05ng/mL. |
| Gene Symbol: |
IGFBP3 |
| Gene ID: |
3486 |
| Uniprot ID: |
IBP3_HUMAN |
| Immunogen: |
Recombinant Human IGFBP-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly28-Lys291) of human IGFBP3/BP-53 (Accession #NP_000589.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
GASSAGLGPVVRCEPCDARA LAQCAPPPAVCAELVREPGC GCCLTCALSEGQPCGIYTER CGSGLRCQPSPDEARPLQAL LDGRGLCVNASAVSRLRAYL LPAPPAPGNASESEEDRSAG SVESPSVSSTHRVSDPKFHP LHSKIIIIKKGHAKDSQRYK VDYESQSTDTQNFSSESKRE TEYGPCRREMEDTLNHLKFL NVLSPRGVHIPNCDKKGFYK KKQCRPSKGRKRGFCWCVD |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6803211Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedNucleus |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 3 proteinIbp-3 proteinIgf-Binding Protein 3 proteinIgfbp-3 proteinIGFBP3 proteinIBP3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
