Host: | HEK293 |
Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Recombinant-Human GUCY2C/Guanylyl cyclase C-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
Conjugation: | Biotin |
Formulation: | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
Dilution Range: | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
Storage Instruction: | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
Gene Symbol: | GUCY2C |
Gene ID: | 2984 |
Uniprot ID: | GUC2C_HUMAN |
Immunogen Region: | Ser24-Gln430 |
Immunogen: | DNA sequence encoding Human GUCY2C/Guanylyl cyclase C including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
Function | Guanylyl cyclase that catalyzes synthesis of cyclic GMP (cGMP) from GTP. Receptor for the E.coli heat-stable enterotoxin.E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GUCY2C. Also activated by the endogenous peptides guanylin and uroguanylin. |
Protein Name | Guanylyl Cyclase CGc-CHeat-Stable Enterotoxin ReceptorSta ReceptorHstarIntestinal Guanylate Cyclase |
Database Links | Reactome: R-HSA-8935690Reactome: R-HSA-8942233 |
Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneThe 145 Kda Plasma Membrane Form Of Gucy2c Contains Sialic Acid And Galactose ResiduesWhile A Differencially Glycosylated 130 Kda Form Is A High Mannose Form That Is Resident In The Endoplasmic Reticulum And May Serve As The Precursor For The Cell Surface Form |
Alternative Protein Names | Guanylyl Cyclase C proteinGc-C proteinHeat-Stable Enterotoxin Receptor proteinSta Receptor proteinHstar proteinIntestinal Guanylate Cyclase proteinGUCY2C proteinGUC2C proteinSTAR protein |
Information sourced from Uniprot.org