Human GDF2 (Growth Differentiation Factor 2) Sandwich ELISA Kit (STJE0019696)

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STJE0019696
£163.50 - £357.50
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25-2000pg/mL
Short Description : This GDF2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GDF2
Gene ID: 2658
Uniprot ID: GDF2_HUMAN
Specificity: This kit recognizes Human GDF2 in samples. No significant cross-reactivity or interference between Human GDF2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications A reversible disulfide bond can be formed between the two subunits in the homodimer.this has no effect on GDF2 activity.
Function Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.
Protein Name Growth/Differentiation Factor 2
Gdf-2
Bone Morphogenetic Protein 9
Bmp-9
Database Links Reactome: R-HSA-201451
Cellular Localisation Secreted
Alternative ELISA Names Growth/Differentiation Factor 2 ELISA kit
Gdf-2 ELISA kit
Bone Morphogenetic Protein 9 ELISA kit
Bmp-9 ELISA kit
GDF2 ELISA kit
BMP9 ELISA kit
Specificity This kit recognizes Human GDF2 in samples. No significant cross-reactivity or interference between Human GDF2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-111 102
EDTA plasma (n=8) 90-102 96
Cell culture media (n=8) 94-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.08 227.51 770.01 88.66 215.59 811.08
Standard deviation 6.55 12.35 25.80 5.01 9.01 37.15
CV (%) 6.96 5.43 3.35 5.65 4.18 4.58

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