| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.2 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.01 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.1-0.4 Mu g/mL in the presence of 7.5 ng/mL of rhActivin A. |
| Gene Symbol: | FST |
| Gene ID: | 10468 |
| Uniprot ID: | FST_HUMAN |
| Immunogen Region: | Gly30-Asn317 |
| Immunogen: | Recombinant Human Follistatin/Follistatin 288/FST Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly30-Asn317) of human Follistatin/Follistatin 288/FST (Accession #P19883) with a hFC at the N-ter |
| Immunogen Sequence: | GNCWLRQAKNGRCQVLYKTE LSKEECCSTGRLSTSWTEED VNDNTLFKWMIFNGGAPNCI PCKETCENVDCGPGKKCRMN KKNKPRCVCAPDCSNITWKG PVCGLDGKTYRNECALLKAR CKEQPELEVQYQGRCKKTCR DVFCPGSSTCVVDQTNNAYC VTCNRICPEPASSEQYLCGN DGVTYSSACHLRKATCLLGR SIGLAYEGKCIKAKSCEDIQ CTGGKKCLWDFKVGRGRCS |
| Function | Multifunctional regulatory protein whose primary function is to antagonize members of the transforming growth factor beta (TGF-beta) superfamily including activin, myostatin, GDF11 or bone morphogenetic proteins (BMPs). Mechanistically, binds to these ligands in the extracellular space, blocking their type II receptor-binding site to inhibit downstream signaling. Plays an essential role in muscle fiber formation and growth both by preventing the repressive effects of myostatin and through SMAD3/AKT/mTOR signaling independently of myostatin. Also promotes neural differentiation by antagonizing the action BMP4. Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) by sequestering activin A/INHBA. On the other hand, translocates into the nucleus where it down-regulates rRNA synthesis and ribosome biogenesis to maintain cellular energy homeostasis by binding to rDNA. |
| Protein Name | FollistatinFsActivin-Binding Protein |
| Database Links | Reactome: R-HSA-2473224 |
| Cellular Localisation | SecretedNucleusNucleolus |
| Alternative Protein Names | Follistatin proteinFs proteinActivin-Binding Protein proteinFST protein |
Information sourced from Uniprot.org
