| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Fas Ligand at 2 Mu g/mL (100 Mu L/well) can bind recombinant Human FAS. The EC50 of Human FAS is 6.23 ng/mL.|2.Measured by its ability to inhibit Fas Li |
| Gene Symbol: | FAS |
| Gene ID: | 355 |
| Uniprot ID: | TNR6_HUMAN |
| Immunogen Region: | Gln26-Asn173 |
| Immunogen: | Recombinant Human FAS/APO-1/CD95 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Asn173) of human FAS/CD95/APO-1/TNFRSF6 (Accession #NP_000034.1) fused with an Fc, 6×His tag at the C-terminu |
| Immunogen Sequence: | QVTDINSKGLELRKTVTTVE TQNLEGLHHDGQFCHKPCPP GERKARDCTVNGDEPDCVPC QEGKEYTDKAHFSSKCRRCR LCDEGHGLEVEINCTRTQNT KCRCKPNFFCNSTVCEHCDP CTKCEHGIIKECTLTSNTKC KEEGSRSN |
| Post Translational Modifications | (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 6 Apo-1 Antigen Apoptosis-Mediating Surface Antigen Fas Faslg Receptor Cd Antigen Cd95 |
| Database Links | Reactome: R-HSA-140534 Reactome: R-HSA-3371378 Reactome: R-HSA-5213460 Reactome: R-HSA-5218900 Reactome: R-HSA-6803211 Reactome: R-HSA-69416 Reactome: R-HSA-75157 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft Isoform 2: Secreted Isoform 3: Secreted Isoform 4: Secreted Isoform 5: Secreted Isoform 6: Secreted |
| Alternative Protein Names | Tumor Necrosis Factor Receptor Superfamily Member 6 protein Apo-1 Antigen protein Apoptosis-Mediating Surface Antigen Fas protein Faslg Receptor protein Cd Antigen Cd95 protein FAS protein APT1 protein FAS1 protein TNFRSF6 protein |
Information sourced from Uniprot.org