Human FAS/APO-1/CD95 protein (Recombinant) (C-hFc & His) (STJP002580)

SKU:
STJP002580

£80.50 - £257.50
Current Stock:
Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.1 EU/Mu g of the protein by LAL method.
Immunoreactivity: 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human Fas Ligand at 2 Mu g/mL (100 Mu L/well) can bind recombinant Human FAS. The EC50 of Human FAS is 6.23 ng/mL.|2.Measured by its ability to inhibit Fas Li
Gene Symbol: FAS
Gene ID: 355
Uniprot ID: TNR6_HUMAN
Immunogen Region: Gln26-Asn173
Immunogen: Recombinant Human FAS/APO-1/CD95 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Asn173) of human FAS/CD95/APO-1/TNFRSF6 (Accession #NP_000034.1) fused with an Fc, 6×His tag at the C-terminu
Immunogen Sequence: QVTDINSKGLELRKTVTTVE TQNLEGLHHDGQFCHKPCPP GERKARDCTVNGDEPDCVPC QEGKEYTDKAHFSSKCRRCR LCDEGHGLEVEINCTRTQNT KCRCKPNFFCNSTVCEHCDP CTKCEHGIIKECTLTSNTKC KEEGSRSN
Post Translational Modifications (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 6
Apo-1 Antigen
Apoptosis-Mediating Surface Antigen Fas
Faslg Receptor
Cd Antigen Cd95
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75157
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Protein Names Tumor Necrosis Factor Receptor Superfamily Member 6 protein
Apo-1 Antigen protein
Apoptosis-Mediating Surface Antigen Fas protein
Faslg Receptor protein
Cd Antigen Cd95 protein
FAS protein
APT1 protein
FAS1 protein
TNFRSF6 protein

Information sourced from Uniprot.org

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