• Human F8 (Coagulation factor VIII) CLIA Kit (STJC0000433)

Human F8 (Coagulation factor VIII) CLIA Kit (STJC0000433)

SKU:
STJC0000433

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human F8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F8
Gene ID: 2157
Uniprot ID: FA8_HUMAN
Specificity: This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Sulfation on Tyr-1699 is essential for binding vWF. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.
Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Protein Name Coagulation Factor Viii
Antihemophilic Factor
Ahf
Procoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-140875
Reactome: R-HSA-163841
Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-9672383
Reactome: R-HSA-9672387
Reactome: R-HSA-9672391
Reactome: R-HSA-9672393
Reactome: R-HSA-9672395
Reactome: R-HSA-9672396
Reactome: R-HSA-9672397
Reactome: R-HSA-9673202
Reactome: R-HSA-9674519
Cellular Localisation Secreted
Extracellular Space
Alternative CLIA Names Coagulation Factor Viii CLIA kit
Antihemophilic Factor CLIA kit
Ahf CLIA kit
Procoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain CLIA kit
F8 CLIA kit
F8C CLIA kit
Specificity This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 99
EDTA plasma(n=8) 88-102 94
Cell culture media(n=8) 89-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 211.11 377.50 1679.54 230.89 360.33 1787.94
Standard deviation 23.39 31.94 144.10 19.26 32.25 132.49
CV (%) 11.08 8.46 8.58 8.34 8.95 7.41
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance