• Human F7 (Coagulation factor VII) CLIA Kit (STJC0000392)

Human F7 (Coagulation factor VII) CLIA Kit (STJC0000392)

SKU:
STJC0000392

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human F7 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: F7
Gene ID: 2155
Uniprot ID: FA7_HUMAN
Specificity: This kit recognizes Human F7 in samples. No significant cross-reactivity or interference between Human F7 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Plasma.
Post Translational Modifications The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-S/T-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Can be either O-glucosylated or O-xylosylated at Ser-112 by POGLUT1 in vitro.
Function Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
Protein Name Coagulation Factor Vii
Proconvertin
Serum Prothrombin Conversion Accelerator
Spca
Eptacog Alfa Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain
Database Links Reactome: R-HSA-1368108
Reactome: R-HSA-140834
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Cellular Localisation Secreted
Alternative CLIA Names Coagulation Factor Vii CLIA kit
Proconvertin CLIA kit
Serum Prothrombin Conversion Accelerator CLIA kit
Spca CLIA kit
Eptacog Alfa Cleaved Into - Factor Vii Light Chain - Factor Vii Heavy Chain CLIA kit
F7 CLIA kit
Specificity This kit recognizes Human F7 in samples. No significant cross-reactivity or interference between Human F7 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-109 101
EDTA plasma(n=8) 90-106 97
Cell culture media(n=8) 91-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 53.63 105.07 393.82 55.69 101.27 363.99
Standard deviation 4.37 8.80 27.92 6.20 10.84 24.53
CV (%) 8.15 8.38 7.09 11.13 10.70 6.74
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance