Human EPRS1 protein (Recombinant) (N-His) (STJP006559)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human EPRS1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | EPRS1 |
| Gene ID | 2058 |
| Uniprot ID | SYEP_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp749-Thr956 |
Additional Info
| Function | Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin. The GAIT complex inhibits the translation of these mRNAs, allowing interferon-gamma to redirect the function of EPRS1 from protein synthesis to translation inhibition in specific cell contexts. Furthermore, it can function as a downstream effector in the mTORC1 signaling pathway, by promoting the translocation of SLC27A1 from the cytoplasm to the plasma membrane where it mediates the uptake of long-chain fatty acid by adipocytes. Thereby, EPRS1 also plays a role in fat metabolism and more indirectly influences lifespan. |
| Protein Name | Bifunctional Glutamate/Proline--Trna LigaseBifunctional Aminoacyl-Trna SynthetaseCell Proliferation-Inducing Gene 32 ProteinGlutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna LigaseGlutamyl-Trna SynthetaseGlurs - Proline--Trna LigaseProlyl-Trna Synthetase |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-6782315Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane ProteinTranslocates From Cytosol To Membranes Upon Phosphorylation At Ser-999 |
| Alternative Protein Names | Bifunctional Glutamate/Proline--Trna Ligase proteinBifunctional Aminoacyl-Trna Synthetase proteinCell Proliferation-Inducing Gene 32 Protein proteinGlutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna Ligase proteinGlutamyl-Trna Synthetase proteinGlurs - Proline--Trna Ligase proteinProlyl-Trna Synthetase proteinEPRS1 proteinEPRS proteinGLNS proteinPARS proteinQARS proteinQPRS proteinPIG32 protein |
Information sourced from Uniprot.org