Human EpCAM (EpCAM) Antibody Pair (STJAP00755)
General Information
| Short Description | This EpCAM antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R01-6H2/R04-3C3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EPCAM |
| Gene ID | 4072 |
| Uniprot ID | EPCAM_HUMAN |
| Immunogen | Recombinant protein of human EpCAM |
Additional Info
| Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. |
| Post Translational Modifications | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Protein Name | Epithelial Cell Adhesion MoleculeEp-CamAdenocarcinoma-Associated AntigenCell Surface Glycoprotein Trop-1Epithelial Cell Surface AntigenEpithelial GlycoproteinEgpEpithelial Glycoprotein 314Egp314Hegp314Ks 1/4 AntigenKsaMajor Gastrointestinal Tumor-Associated Protein Ga733-2Tumor-Associated Calcium Signal Transducer 1Cd Antigen Cd326 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Lateral Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionTight JunctionColocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction |
| Alternative Antibody Pair Names | Epithelial Cell Adhesion Molecule antibody pairEp-Cam antibody pairAdenocarcinoma-Associated Antigen antibody pairCell Surface Glycoprotein Trop-1 antibody pairEpithelial Cell Surface Antigen antibody pairEpithelial Glycoprotein antibody pairEgp antibody pairEpithelial Glycoprotein 314 antibody pairEgp314 antibody pairHegp314 antibody pairKs 1/4 Antigen antibody pairKsa antibody pairMajor Gastrointestinal Tumor-Associated Protein Ga733-2 antibody pairTumor-Associated Calcium Signal Transducer 1 antibody pairCd Antigen Cd326 antibody pairEPCAM antibody pairGA733-2 antibody pairM1S2 antibody pairM4S1 antibody pairMIC18 antibody pairTACSTD1 antibody pairTROP1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human EpCAM (EpCAM) Antibody Pair (STJAP00755)
This antibody pair detects human EpCAM (UniProt P16422) and is validated for human samples only. Both antibodies were raised against a recombinant protein of human EpCAM.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. The clone IDs are R01-6H2 and R04-3C3 respectively.
This antibody pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Working dilutions are not pre-defined for this product and must be determined by the end user. Titration experiments are recommended to establish optimal concentrations for each specific experimental setup.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquots should be kept at -20°C, and repeated freeze/thaw cycles should be avoided to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.