| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human ENPP7 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ENPP7 |
| Gene ID: |
339221 |
| Uniprot ID: |
ENPP7_HUMAN |
| Specificity: |
This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon. |
| Post Translational Modifications | N-glycosylated.required for activity and transport to the plasma membrane. |
| Function | Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Has also phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen. |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7E-Npp 7Npp-7Alkaline Sphingomyelin PhosphodiesteraseIntestinal Alkaline SphingomyelinaseAlk-Smase |
| Database Links | Reactome: R-HSA-1660662 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinThe Catalytic Domain Is Released Into The Extracellular Medium When Cells Are Treated With TrypsinLocalized At The Surface Of The Microvillar Membrane In Small Intestine EnterocytesAnd In Endosome-Like Structures Situated Beneath The Microvillar MembraneAnd In Golgi Complex |
| Alternative CLIA Names | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7 CLIA kitE-Npp 7 CLIA kitNpp-7 CLIA kitAlkaline Sphingomyelin Phosphodiesterase CLIA kitIntestinal Alkaline Sphingomyelinase CLIA kitAlk-Smase CLIA kitENPP7 CLIA kitUNQ3077 CLIA kitPRO9912 CLIA kit |
| Specificity | This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 100 |
| EDTA plasma(n=8) | 91-102 | 97 |
| Cell culture media(n=8) | 100-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 237.78 | 485.98 | 2153.04 | 259.83 | 511.81 | 1965.23 |
| Standard deviation | 20.95 | 44.37 | 168.15 | 27.05 | 45.86 | 136.78 |
| CV (%) | 8.81 | 9.13 | 7.81 | 10.41 | 8.96 | 6.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
