Human E2-25K/UBE2K protein (Recombinant) (His-Tag) (STJP017032)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human E2-25K/UBE2K-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% Glycerol, 50mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2K |
| Gene ID | 3093 |
| Uniprot ID | UBE2K_HUMAN |
| Accession Number | NP_005330 |
| Immunogen | Human |
| Immunogen Region | 1-200aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. |
| Post Translational Modifications | Sumoylation at Lys-14 impairs catalytic activity. |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B.in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 KE2 Ubiquitin-Conjugating Enzyme KHuntingtin-Interacting Protein 2Hip-2Ubiquitin Carrier ProteinUbiquitin-Conjugating Enzyme E2-25 KdaUbiquitin-Conjugating Enzyme E2(25kUbiquitin-Conjugating Enzyme E2-25kUbiquitin-Protein Ligase |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-936440Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 K proteinE2 Ubiquitin-Conjugating Enzyme K proteinHuntingtin-Interacting Protein 2 proteinHip-2 proteinUbiquitin Carrier Protein proteinUbiquitin-Conjugating Enzyme E2-25 Kda proteinUbiquitin-Conjugating Enzyme E2(25k proteinUbiquitin-Conjugating Enzyme E2-25k proteinUbiquitin-Protein Ligase proteinUBE2K proteinHIP2 proteinLIG protein |
Information sourced from Uniprot.org